Anthelmintic Screening of Phytomedicines using Haemonchus placei Adult Motility Assay



Background: The leaf extract of S. mombin was previously shown to be active against gastrointestinal nematodes (egg and infective larvae) in sheep.
Objective: To examine S. mombin extracts against adult H. placei, a cattle parasite and optimise the adult worm motility assay.
Materials and Method: Levamisole hydrochloride was used to optimise incubation time in the assay protocol. Adult worms (ten) were incubated, in duplicate, with standard drug solutions in either phosphate-buffered saline, pH 7.4 (PBS-7.4) or normal saline (NS) for 3h. Worms were afterwards examined and classified as dead or paralysed. The activities of three S. mombin leaf extracts (AH-H01, AH-A02, AH-W03) were investigated and median lethal concentration (LC50) was estimated by sigmoidal curve-fitting analysis.
Results: Three hours incubation time was found optimal. Significantly, NS was better than PBS-7.4 in solvating plant extracts, which gave LC50 values of (AH-H01; 104 mg/mL), (AH-A02; 30.5 mg/mL) and (AH-W03; 56.27 mg/mL) respectively.
Conclusion: S. mombin extracts are active against adult H. placei and the optimised assay enhanced sample throughput in anthelmintic phytomedicine screening.
Keywords: Screening assay, Heamonchus placei, Anthelminitics, Spondias mombin, Plant extracts

How to Cite
OGBEDENGBE, A N; IDOWU, S O; ADEMOLA, I O. Anthelmintic Screening of Phytomedicines using Haemonchus placei Adult Motility Assay. Nigerian Journal of Pharmaceutical Research, [S.l.], v. 15, n. 1, p. 75-83, july 2019. ISSN 2635-3555. Available at: <>. Date accessed: 07 june 2020.

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